Gene transfer in Acinetobacter calcoaceticus NCIB8250

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Serotyping of Acinetobacter calcoaceticus.

Serotyping of Acinetobacter calcoaceticus by direct immunofluorescence and a capsule swelling reaction is described. One hundred isolates, including 12 from an outbreak in a neonatal department, were studied. Ninety five per cent of the isolates were typable by immunofluorescence and could be divided into 30 separate types, but 42.1% of typable strains, including 11 from the outbreak, were of o...

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Gene transfer in Acinetobacter calcoaceticus: fertility variants of the sex factor pAV1.

The naturally occurring transmissible plasmid pAV1 mediates chromosome transfer and can exhibit two distinct levels of transmissibility in Acinetobacter calcoaceticus strain EBF65/65. The two states of pAV1 have been arbitrarily designated pAV1a (low frequency variant) and pAV1b (high frequency variant). Both variants have the same incompatibility and host range properties and each mobilizes tw...

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Chromosome mapping in Acinetobacter calcoaceticus.

RP4-mediated conjugation was used to map the loci of 23 different mutations on a circular linkage group in Acinetobacter calcoaceticus EBF65/65. The resulting genetic map indicated that the chromosomal organization of A. calcoaceticus differed from that of members of the enteric group of bacteria and was similar to Pseudomonas in showing an absence of clustering of functionally related genes.

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Defining resistance in Acinetobacter calcoaceticus-Acinetobacter baumannii complex strains.

We read with great interest the recent publication of Tien and colleagues in which they describe the antimicrobial susceptibilities of a large number of Acinetobacter baumannii-Acinetobacter calcoaceticus strains isolated from a medical center in Taiwan (1). They suggested that antimicrobial susceptibility was negatively associated with virulent potential because more-susceptible strains were r...

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Nucleotide sequence of the Acinetobacter calcoaceticus trpGDC gene cluster.

A plasmid library of Acinetobacter calcoaceticus HindIII fragments was constructed, and clones that complemented an Escherichia coli pabA mutant were selected. Plasmids containing a 3.9-kb fragment of A. calcoaceticus DNA that also complemented E. coli trpD and trpC-(trpF+) mutants were obtained. We infer that complementation of E. coli pabA mutants was the result of the expression of the amphi...

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ژورنال

عنوان ژورنال: FEMS Microbiology Letters

سال: 1985

ISSN: 0378-1097

DOI: 10.1016/0378-1097(85)90414-8